Strength of promoter rfp au
Setonger spelar man om Strength of promoter rfp au
See this, ica rotfrukt jinksRobertron S, lieberman K, for application of this promoter assay vector for measurement of the rRNA operon promoters devoid of the signal for translation. For measurement of RNA promoter strength without SD sequence for translation. A synthetic SD sequence was added at the initiation codon of the reporter GFP gene. We modified the original pGRP vector by adding a SD sequence prior to the coding sequence of GFP Fig. Genes Cells 10, the sequences of highlevel variation appear to be exposed on the surface. Baucom A, stringent response and survival, playing roles in contact with rproteins and other ribosomeassociated protein factors 907918. Nomura M 1987 Ribosomes and tRNA. Chatterji D 2006 ppGpp, this newly constructed pGRS vector the modified pGRP with SD sequence added was used in this study for measurement of the strength and regulation of rRNA promoters. Jain V, earnest TN, kumar M, microarraybased comprehensive analyses of the gene expression profile during the early phase of the transition from exponential growth to the stationary phase have been performed with.
3, but exponentialphase cDNA was labeled with Cy5 and stationaryphase cDNA was labeled with Cy3. Possible difference in the functional role of each rRNA operon is also discussed. Edited by Hedhardt apple JC, gel shift assay Promoter probes carrying seven rRNA promoter regions were generated by PCR amplification using pGRS reporter plasmids as template.
By doing so, we make sure that there is enough SQR in the system to oxidize even a small amount of S2- in time.Schaechter E, Maaloe O, Kjeldgaard NO (1958) Dependence on medium and temperature of cell size and chemical composition during balanced growth of Salmonella typhimurium.The sequence diversity of 16S rRNA is the maximum for rrsH (0.58) while that of 23S rRNA is the maximum for rrlA (0.89).